Changing Cross-Reactivity for Different Immunoassays Using the Same Antibodies: Theoretical Description and Experimental Confirmation
نویسندگان
چکیده
Many applications of immunoassays involve the possible presence structurally similar compounds that bind with antibodies, but different affinities. In this regard, an important characteristic immunoassay is its cross-reactivity: possibility detecting various in comparison a certain standard. Based on cross-reactivity, analytical systems are assessed as either high-selective (responding strictly to specific compound) or low-selective number compounds). The present study demonstrates cross-reactivity not intrinsic antibodies can vary for formats competitive using same antibodies. Assays sensitive detection markers and, accordingly, implementation at low concentrations and modified (competing) antigens characterized by lower cross-reactivities are, thus, more than assays requiring high interacting reagents. This effect was confirmed both mathematical modeling experimental enzyme fluorescence polarization sulfonamides fluoroquinolones. Thus, shifting reagents decreases up five-fold. Moreover, changed even assay format varying ratio immunoreactants’ from kinetic equilibrium mode antigen-antibody reaction. described patterns demonstrate modulating immunodetection selectivity without searching new binding reactants.
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ژورنال
عنوان ژورنال: Applied sciences
سال: 2021
ISSN: ['2076-3417']
DOI: https://doi.org/10.3390/app11146581